SubtiBank SubtiBank
yfkN [2017-8-11 16:0:3]
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yfkN [2017-8-11 16:0:3]

cell wall-anchored 3'5' cyclic dinucleotide 3' phosphodiesterase, degrades c-di-AMP
Locus
BSU07840
Isoelectric point
5.02
Molecular weight
159.47 kDa
Protein length
1462 aa Sequence Blast
Gene length
4386 bp Sequence Blast
Function
degradation of c-di-AMP
Product
cell wall-anchored 3'5' cyclic dinucleotide 3′ phosphodiesterase, degrades c-di-AMP
Essential
no
E.C.
3.1.4.16
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Nucleotide metabolism → Metabolism of signalling nucleotides
  • Groups of genes → Secreted proteins

    • Gene

    Coordinates
    855,114 → 859,502

    Phenotypes of a mutant

  • essential according to Kobayshi et al. PubMed, but a deletion mutant could be constructed PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • degrades c-di-AMP two 5′ AMP molecules PubMed

    • Paralogous protein(s)

  • YhcR

    • Cofactors

  • Mn2+ PubMed

    • Structure

  • 3GVE

    • Localization

  • extracellular (signal peptide) PubMed, the transmembrane domain serves as retention signal, attached to the cell wall PubMed

    • Expression and Regulation

    Operons

    Genes
    yfkN
    Description
    PubMed

    Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • PhoP: activation, PubMed, in PhoP regulon

    • Regulation

  • expressed under conditions of phosphate limitation (PhoP) PubMed
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    Biological materials

    Mutant

  • GP2026 (ΔyfkN::aphA3), available in Jörg Stülke's lab PubMed
  • MGNA-C263 (yfkN::erm), available at the NBRP B. subtilis, Japan

    • References

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